OB Cadherin (CDH11) Mouse Monoclonal Antibody [Clone ID: 16A]

Specifications

Product Data
Clone Name	16A
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen Sections: Use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended Dilutions: 1/25-1/50 for Immunohistochemistry with Avidin-biotinylated Horseradish Peroxidase Complex (ABC) as detection reagent and 1/25-1/250 for Immunoblotting applications.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Affinity purified extracellular domain of Human Cadherin-11-GST fusion protein.
Specificity	This antibody recognizes the extracellular domain of Cadherin-11.
Formulation	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cadherin 11
Database Link	Entrez Gene 1009 Human UniProt ID P55287
Background	Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
Synonyms	Osteoblast cadherin, OSF-4, CDH11, OB Cadherin
Reference Data

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