Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: RGE53]

Specifications

Product Data
Clone Name	RGE53
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunoblotting: 1/100-1/1000. Immunocytochemistry. Flow Cytometry. Immunohistochemistry on Frozen tissues: 1/100-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reactivity	Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal preparation of cells.
Specificity	Clone RGE53 reacts exclusively with cytokeratin 18 which is present in glandular epithel cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratins are a subfamily of intermediatefilament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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