Cytokeratin 4 (KRT4) Mouse Monoclonal Antibody [Clone ID: 6B10]

Specifications

Product Data
Clone Name	6B10
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunocytochemistry (Permeabilized cells).   Immunohistochemistry on Frozen tissues (1/25-1/200) using with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western blot (1/100-1/1000). Flow Cytometry (1/25-1/200).
Reactivity	Canine, Feline, Human, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytokeratin preparation extracted from Human esophagus.
Specificity	This Monoclonal antibody 6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 4
Database Link	Entrez Gene 3851 Human UniProt ID P19013
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Synonyms	KRT4, CYK4, Cytokeratin-4, CK4, Keratin-4, K4
Reference Data

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