Cytokeratin 3 (KRT3) Mouse Monoclonal Antibody [Clone ID: AE5]

Specifications

Product Data
Clone Name	AE5
Applications	IHC, WB
Recommend Dilution	Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
Reactivity	Bovine, Human, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epithelial keratin
Specificity	Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3; formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.
Formulation	PBS, pH 7,2 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ion Exchange Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 3
Database Link	Entrez Gene 3850 Human UniProt ID P12035
Background	Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.
Synonyms	keratin-3, keratin-76, KRT3, Type-II keratin Kb3
Reference Data

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