Actin (ACTA1) Mouse Monoclonal Antibody [Clone ID: 647]

Specifications

Product Data
Clone Name	647
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunofluorescence. Immunoblotting: One single band is reactive corresponding to Actin.  Working Dilution: 1/5-1/10. Dilute immediately before use with PBS or TBS. Recommended Positive Control: Muscle or myofibrils.
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Antigen isolated from Chicken skeletal muscle cells
Specificity	This Monoclonal antibody 647 is useful for studying the intracellular distribution and structure of Actin in the cytoskeletal system.
Formulation	State: Supernatant State: Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	actin, alpha 1, skeletal muscle
Database Link	Entrez Gene 58 Human UniProt ID P68133
Background	The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
Synonyms	ACTA; Alpha-actin-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3
Reference Data

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