DNA Mouse Monoclonal Antibody [Clone ID: AC-30-10]

Specifications

Product Data
Clone Name	AC-30-10
Applications	IF, IHC
Recommend Dilution	Cell Culture. Dot Blot on nitrocellulose membrane (after baking at 70°C). Immunohistochemistry on Frozen and Paraffin Embedded Tissue. Working Dilution: After reconstitution with 1 ml distilled water, dilute further 1/10 with PBS, pH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Double- and single-stranded DNA
Specificity	Antibody clone AC-30-10 is a reliable Positive Control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. It can also be used for sensitive detection of mycoplasma contamination in cell cultures. Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. The antibody recognizes DNA in all DNA-containing species. Tested Reactivities on Cultured Cell Lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.
Formulation	PBS, pH 7.4 State: Purified State: Lyophilized purified IgM fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore in 1 ml distilled water
Purification	Gel Filtration
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Synonyms	Deoxyribonucleic Acid
Reference Data

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