Uroplakin III (UPK3A) Mouse Monoclonal Antibody [Clone ID: AU1]

Specifications

Product Data
Clone Name	AU1
Applications	IHC, WB
Recommend Dilution	Immunoblotting (Western blotting). Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 for 1h at RT using a heat-induced retrieval method (e.g. microwave treatment). Not Suitable for Frozen Sections!.
Reactivity	Bovine, Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Asymmetric unit membrane (AUM) preparation from bovine urinary bladder.
Specificity	Clone AU1 reacts specifically with Uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of Uroplakin III. Until now, no Uroplakin staining was found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.
Formulation	Final solution contains PBS, pH 7.4 with 0.5% BSA as stabilizer and 0.09% Sodium Azide as preservative. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein A.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	uroplakin 3A
Database Link	Entrez Gene 7380 Human UniProt ID O75631
Background	Together with the Uroplakins UP Ia, UP Ib and UP II, Uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994).
Synonyms	UP3a, Uroplakin III, UPIII, UPK3
Reference Data

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