Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: V9]

Specifications

Product Data
Clone Name	V9
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunoblotting. Flow Cytometry. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions: 1/25–1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Reactivity	Chicken, Human, Rat, Sheep, Potoroo
Host	Mouse
Clonality	Monoclonal
Immunogen	V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.
Specificity	The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Formulation	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Synonyms	VIM
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.