Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: Ks8.7]

Specifications

Product Data
Clone Name	Ks8.7
Applications	IF, IHC, WB
Recommend Dilution	Western blot. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections (After microwave treatment). Recommended Dilutions: When reconstituted with 1 ml distilled water, dilute further 1/10 for immunohistochemistry. Incubation time: 1 h at RT.
Reactivity	Hamster, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal proteins from cultured HeLa cells
Specificity	Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypetide Reacting: Mr 52500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia. Tumors Specifically Detected: All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA. Tested Reactivities on cultured cell lines: MCF-7, RT 112, PLC.
Formulation	Final solution contains 0.09% Sodium Azide and 0.5% BSA in PBS buffer, pH 7.4 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 1 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20.  The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.  Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data

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