SCF (KITLG) Mouse Monoclonal Antibody [Clone ID: hKL12]

Specifications

Product Data
Clone Name	hKL12
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 0.15 µg/ml as a titer in ELISA against the recombinant antigen. Has been described to work in Wetsern blots and Immunohistochemistry.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human stem cell factor.
Specificity	This Monoclonal antibody hKL12 is specific for Stem Cell Factor (hSCF). hKL12 is useful for the detection of hSCF in tissues, isolated cells and for studying biological effects of Human Stem Cell Factor in vitro. Antigen distribution on Tissue sections: Bone marrow stromal cells, fibroblasts, and fetal liver cells express SCF. Keratinocytes stain positive.
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore by adding 0.5 ml distilled water.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	KIT ligand
Database Link	Entrez Gene 4254 Human UniProt ID P21583
Background	SCF is a transmembrane protein expressed as two alternatively spliced isoforms, either 220 or 248 amino acids, the former lacking a peptidase cleavage site. The cleavage site is responsible for the release of the soluble form of the protein from the cell surface. The first 164 or 165 residues of the extracellular domain comprise soluble SCF, which exists in solution as a noncovalently linked homodimer. Alone, SCF is a weakly proliferative stimulus but acts synergistically with numerous cytokines. SCF is important in germ cell developement, and an indispensable facor for mast cell proliferation an differentiation.
Synonyms	KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer

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