CCN2 Mouse Monoclonal Antibody [Clone ID: 2154-60]

Specifications

Product Data
Clone Name	2154-60
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 2 µg/ml (1/500). Immunohistochemistry on Paraffin Embedded Sections: 20 µg/ml (1/50). Microwave pretreatment in citrate buffer is recommended for antigen retrieval. Suggested Positive Control: Human placenta, Rat spleen.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human CTGF
Specificity	This antibody detects CTGF. This monoclonal antibody also stains cells in Rat spleen, indicating significant cross reactivity with the corresponding Rat CTGF.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified Tissue Culture Supernatant Stabilizer: None Preservative: 0.05% Kathon
Concentration	1.0 mg/ml
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	connective tissue growth factor
Database Link	Entrez Gene 1490 Human UniProt ID P29279
Background	Connective Tissue Growth Factor (CTGF) is an immediate early growth responsive gene that has been shown to be a downstream mediator of TGFbeta actions in fibroblasts and vascular endothelial cells. CTGF promotes cell adhesion and mitogenesis in both fibroblasts and endothelial cells and stimulates cell migration in fibroblasts. The localization of CTGF in angiogenic tissues, as well as in atherosclerotic plaques, suggests a possible role in the regulation of vessel growth during development, wound healing, and vascular disease. CTGF is expressed in myocardial infarct tissue following acute myocardial infarction.
Synonyms	CCN2, HCS24, IGFBP8, Connective tissue growth factor, IGF-binding protein 8,IBP8
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer’s hemalum
Reference Data

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