MHC Class II (I-A k,s,r) Rat Monoclonal Antibody [Clone ID: ER-TR2]

Specifications

Product Data
Clone Name	ER-TR2
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200).  Suggested Positive Control: Mouse spleen. Has been described to work in FACS. Does not react on routinely processed paraffin sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one α-chain (31-34kDa) and one β-chain (26-29kDa). The epitope has not been further characterized.
Specificity	This antibody detects cells expressing MHC class II antigens. Monoclonal antibody ER-TR2 is one member of a family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) which detect MHC class II antigens encoded by the murine Ia region of the H-2 complex. They are valuable tools for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). These antibodies also offer new possibilities for studying the development of T helper cells since they also stain stromal cells in the thymus. Isolated cells: The antigen is found on dendritic cells, B-cells and macrophages. The level of antigen detected by ER-TR1, ER-TR2 and ER-TR3 differs from strain to strain (see table on back). Tissue Sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
Formulation	Phosphate buffered saline, pH 7.2 (PBS) State: Purified State: Lyophilized purified Ig fraction Stabilizer: 5 mg/ml BSA Preservative: 0.05% Kathon CG
Reconstitution Method	Restore with 0.5 ml distilled water (=stock solution).
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized product at 2-8 °C. Following reconstitution store (in aliqouts) at -20°C. Avoid repeated freezing and thawing.
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
Reference Data

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