Pecam1 Rat Monoclonal Antibody [Clone ID: ER-MP12]

Specifications

Product Data
Clone Name	ER-MP12
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Acetone Fixed Frozen Sections: 0.4 µg/ml) (1/1600). Immunohistochemistry on Formalin Fixed Paraffin Sections: 15 µg/ml (1/40). Antigen Retrieval with Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 µg/ml.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.
Specificity	Monoclonal antibody ER-MP12 recognizes PECAM-1, platelet / endothelial cell adhesion molecule 1. The antigen is found on major population of colony forming unit macrophage (CFU-M) precursor cells, subpopulation of pre CFU-M and monoblasts, precursor cells of granulocytes. Antigen Distribution on Isolated Cells: The antibody stains the surface of approximately 40% of freshly isolated bone marrow cells of adult mice and the majority of immortalized macrophage precursor cell lines (M1, RMB-1, RMB-3) corresponding to CFU-GM cells. The antibody detects pluri- and multipotent stem cells and prothymocytes as well as particular lymphoid cells in bone marrow and peripheral lymphoid organs. Antigen Distribution in Tissue Sections: The antibody stains the lymphopoietic islands in the spleen of newborn (day 12) and the bone marrow of adult mice. Capillary endothelial cells of adult mice also express the antigen.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: 5 mg/ml BSA Preservative: 0.05% Kathon
Reconstitution Method	Restore with 0.5 ml distilled water (0.6 mg/ml stock solution).
Concentration	0.6 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	platelet/endothelial cell adhesion molecule 1
Database Link	Entrez Gene 18613 Mouse UniProt ID Q08481
Background	Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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