hCG beta (CGB3) Mouse Monoclonal Antibody [Clone ID: H6]

Specifications

Product Data
Clone Name	H6
Applications	ELISA, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: Does not react on routinely processed frozen sections. Immunohistochemistry on Paraffin Sections: 2 µg/ml (1/200). Proteinase K pretreatment for antigen retrieval is recommended. It works also in ELISA. Suggested Positive Control: Human placenta.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This Monoclonal antibody Clone h6 recognizes β-HCG, a well defined oncofetal protein of 36.7 kDa m.w. associated with pregnancy and some cancers. Raised serum levels for HCG are found in teratogenic carcinomas and trophoblastic tumors and occasionally in gastrointestinal, urogenital, bronchial or mammary carcinomas, and in lymphomas.
Formulation	PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	chorionic gonadotropin beta subunit 3
Database Link	Entrez Gene 1082 Human UniProt ID P0DN86
Background	Human gonadothropin is a glycoprotein of 36.7kDa consisting of two subunits. The α-chain (MW 14.5 kD) is common to three other glycoprotein hormones (TSH, FSH, LH) whereas the β-chain (22.2kD) is more different. The β-chain of HCG and LH (lutrophin) have sequence homology in the first 112 amino acids and differ at the C-terminal end consisting of 24 amino acids.
Synonyms	Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700 Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum.
Reference Data

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