ICAM1 Mouse Monoclonal Antibody [Clone ID: 1304]

Specifications

Product Data
Clone Name	1304
Applications	FC, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.25 µg/ml (1/800). Immunohistochemistry on Paraffin Sections: 2 µg/ml (1/100). Pretreatment for antigen retrieval is recommended. Has been decribed to work in FACS and Western Blots.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cultured Human endothelial cells.
Specificity	Clone 1304 detects CD54 (ICAM 1) and is useful for the detection of activated endothelial cells and other activated hemopoietic cells. The antibody inhibits the binding of NK-cells to target cells (K562). Intercellular adhesion molecule (ICAM) 1 or CD54 was initially identified with monoclonal antibody RR1/1 as a widely distributed, cytokine-inducible counter-receptor for lymphocyte-function-associated antigen (LFA) 1 (CD11a/CD18). Granulomas (macrophages/epitheloid cells) in inflamed colon from Crohn's disease and follicular dendritic cells in the tonsil from chronic tonsillitis are strongly positive for CD54 antigen. Also, CD54 is found on activated CD56+/CD3- natural killer cells. Clone 1304 stains a 95kD single chain integral membrane glycoprotein. The epitope is stable against acteone or paraformaldehyde fixation. ICAM 1 also functions as a rhinovirus receptor. The antibody recognizes an epitope on domain 1 of the molecule as tested on transfectants. In inflamed lesions the antibody reacts with stimulated endothelial cells, epithelial cells and fibroblasts and with hemopoietic cells such as macrophages, dendritic cells in Peyer's patches, lymph nodes and tonsils. ICAM 1 expression can be induced on endothelial cells by in vitro stimulation with IL-1, TNFα and IFNγ.
Formulation	PBS pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 3383 Human UniProt ID P05362
Background	ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
Synonyms	ICAM-1
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS 13. Counterstain with Mayer's hemalum
Reference Data

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