CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: 2E1]

Specifications

Product Data
Clone Name	2E1
Applications	ELISA, FC, IHC, IP
Recommend Dilution	Immunohistochemistry on Frozen Sections: 2 µg/ml (1/200). Suggested Positive Control: Human tonsil. Has been described to work in ELISA, Immunoprecipitation and FACS (10 µl stock solution to label 106 cells).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	K562 cells.
Specificity	This Monoclonal Antibody 2E1 recognizes the three isoforms of the Fc-gamma RII designated as A, B and C. Monocytes carry all forms of Fc-gamma RII, Neutrophils carry Fc-gamma RIIA and Fc-gamma RIIC. Fc-gamma RIIB is expressed by B lymphocytes and mast cells.
Formulation	PBS, pH 7.2 containing 0.01% Thiomerosal as preservative and 10 mg/ml BSA as stabilizer State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.5 ml distilled water (stock solution contains 0.4 mg/ml IgG)
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgG receptor IIa
Database Link	Entrez Gene 2212 Human UniProt ID P12318
Background	CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.
Synonyms	Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2
Note	Protocol: Protocol with Frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal Goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated Goat anti Mouse IgG+IgM (H+L) minimal-cross reaction to Human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Reference Data

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