Macrophages Mouse Monoclonal Antibody [Clone ID: PM-2K]

Specifications

Product Data
Clone Name	PM-2K
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/100) Does not react on routinely processed Paraffin Sections. Suggested Positive Control: Human tonsil.
Reactivity	Bovine, Canine, Feline, Human, Monkey, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	24h cultivated Human peritoneal Macrophages
Specificity	This antibody Clone PM-2K, together with X-4 (Cat.-No BM4035) and X-14 (Cat.-No BM4036), forms a particular group of macrophage specific antibodies which were classified at the V Leukocyte Typing Workshop held in Boston in 1993. PM-2K recognises tissue macrophages and macrophages in proliferative disorders of macrophages. PM-2K is one of the markers that, being negative on dendritic cells, can differentiate between macrophages and dendritic cells. Under non-reducing conditions PM-2K recognizes an antigen of 150kD (Western blot) which has been shown to be on cytoplasmic membranes. Antigen Distribution Isolated Cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells; and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue Sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages and macrophages in the interstitial tissues of the kidney, pancreas and many other organs. In Gaucher's disease multicentric reticulohistiocytosis and malignant histiocytosis proliferating macrophages are positive. In MFH infiltrating macrophages are stained but not tumour cells. Some bone stromal cells but not osteoclast-like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are negative.
Formulation	PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% Kathon as a preservative State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Synonyms	Macrophage marker
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Reference Data

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