Ly75 Rat Monoclonal Antibody [Clone ID: NLDC-145]

Specifications

Product Data
Clone Name	NLDC-145
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/400). Recommended Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been described to work in FACS.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse lymph node tissue.
Specificity	Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells. Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts. The antigen is expressed at high levels by dendritic cells and thymic epithelial cells. The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205kDa, also known as DEC-205.
Formulation	PBS, pH 7.2 State: Purified State: Lyophilized purified IgG fraction Stabilizer: 5 mg/ml BSA Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method	Restore by adding 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	lymphocyte antigen 75
Database Link	Entrez Gene 17076 Mouse UniProt ID Q60767
Background	Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.
Synonyms	LY75, CLEC13B, DEC205, gp200-MR6
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Reference Data

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