Desmoglein 2 (DSG2) Mouse Monoclonal Antibody [Clone ID: DG3.10]

Specifications

Product Data
Clone Name	DG3.10
Applications	IF, IHC, WB
Recommend Dilution	Western blotting. Immunofluorescence Microscopy. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment). Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemical application. Incubation time: 1 h at RT, extended with paraffin.
Reactivity	Bovine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	"Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis
Specificity	This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide. Tested Reactivities on Cultured Cell Lines: A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines; Bovine BMGE+H, BMGE-H, MDBK.
Formulation	Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmoglein 2
Database Link	Entrez Gene 1829 Human UniProt ID Q14126
Background	Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.
Synonyms	DSG1, DSG-1, CDHF4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, Desmoglein1, DSG2, DSG-2, CDHF5, HDGC, Desmoglein2
Reference Data
Protein Families	Transmembrane
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC)

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