Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]

Specifications

Product Data
Clone Name	Ks18.04
Applications	IHC, WB
Recommend Dilution	Western blot. Immunohistochemistry on Frozen and Paraffin Sections: 1/20 with PBS. Microwave treatment is recommended. Incubation time: 1h at 37°C, extended with paraffin sections (overnight at 2-8°C).
Reactivity	Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Human keratin K18 from HeLa cytoskeletal preparation
Specificity	Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Tested Reactivities on Cultured Cell Lines: HeLa and MCF-7.
Formulation	PBS, pH 7.4 State: Purified State: Lyophilized purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium azide
Reconstitution Method	Restore in 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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