IGF1 Receptor (IGF1R) (alpha chain) Mouse Monoclonal Antibody [Clone ID: 1H7]

Specifications

Product Data
Clone Name	1H7
Applications	FC, FN, IHC, WB
Recommend Dilution	Functional Assays. Western blotting.  Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Paraffin Sections: 1/10-1/40. This product requires protein digestion pre-treatment of paraffin sections e.g trypsin or pronase. Use pancreas or placenta as histology positive control tissue.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human placental IGF-I receptor.
Specificity	Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (Ref.1). Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (Ref.1)
Formulation	PBS without preservatives or stabilizers State: Low Endotoxin State: Liquid (sterile filtered) purified IgG fraction from tissue culture supernatant
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	insulin like growth factor 1 receptor
Database Link	Entrez Gene 3480 Human UniProt ID P08069
Background	CD221, a 155 kD tyrosine kinase, is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins.
Synonyms	IGF-I receptor, IGF1 Receptor
Reference Data

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