beta V Tubulin (TUBB) (N-term) (Loading Control) Mouse Monoclonal Antibody [Clone ID: TU-06]

Specifications

Product Data
Clone Name	TU-06
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry (paraffin sections): 5 μg/ml, Positive tissue: heart Immunocytochemistry: 2 µg/ml. Staining technique: fixed and permeabilized cells. Positive control: 3T3 mouse embryonal fibroblast cell line Western Blot (reducing conditions): 1 µg/ml, 60 min. Positive control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Beta-subunits of porcine brain tubulin
Specificity	The antibody recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Formulation	Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Precipitation methods; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C. Do not freeze.
Gene Name	tubulin beta class I
Database Link	UniProt ID P07437
Background	The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Synonyms	Tubulin beta chain, Tubulin beta-5 chain
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.