Toxoplasma gondii p30 Mouse Monoclonal Antibody [Clone ID: P30/3]

Specifications

Product Data
Clone Name	P30/3
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: As a guide, the end point titre in an indirect ELISA has been found to be 1/2,000,000 using purified T. gondii P30 as the coating antigen. Western Blot.
Reactivity	Toxoplasma gondii
Host	Mouse
Clonality	Monoclonal
Immunogen	Native Toxoplasma gondii p30 (purified).
Specificity	Reacts with T. gondii P30 membrane protein.
Formulation	PBS buffer, pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q27298
Background	Toxoplasma gondii p30 is the immunodominant surface antigen of Toxoplasma gondii. Toxoplasma gondii is an obligate intracellular protozoan that belongs to the class Sporozoa, and the order Eucoccidia. The parasite has an extremely wide host range and is also nonspecific with respect to the kind of cell type it can enter, making it an interesting model to study invasion. Although toxoplasmosis in healthy adults is generally asymptomatic, it produces severe disease in neonates and immunocompromised people, being the cause of death in many AIDS patients. P30 is the major surface antigen of T. gondii. It is a very abundant protein, conserved in most strains, and present both in the vesicular network of the parasitophorous vacuole and on the surface of the parasite. It is very immunogenic, eliciting high titers of antibodies in infected individuals. The gene coding for P30 has been cloned and completely sequenced: the deduced amino acid sequence shows a putative signal sequence at the N terminus, consistent with localization of P30 on the parasite's surface, as well as a short hydrophobic stretch at the C terminus.
Synonyms	Major surface antigen p30
Reference Data

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