E. coli J5 LPS Mouse Monoclonal Antibody [Clone ID: 2D7/1]

Specifications

Product Data
Clone Name	2D7/1
Applications	ELISA, IF, WB
Recommend Dilution	Immunofluorescence. ELISA: (a 1 mg/ml solution has been used at dilutions of up to 1/1500). Western Blotting.
Reactivity	Escherichia coli
Host	Mouse
Clonality	Monoclonal
Immunogen	Escherichia coli J5 cells.
Specificity	The antibody recognizes E. coli J5 LPS. It has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Synonyms	Escherichia coli, EHEC, EPEC, ETEC
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.