Emcn Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, WB
Recommend Dilution	Western blot: 1-5 μg/ml. FACS: 1-5 μg/ml.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>95%) recombinant Mouse soluble Endomucin (Glu20-Ser190) derived from E. coli (Cat.-No. AR60010PU-N)
Specificity	This antibody detects Mouse Endomucin (Glu20-Ser190). Other species not tested.
Formulation	PBS State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	endomucin
Database Link	Entrez Gene 59308 Mouse UniProt ID Q9R0H2
Background	Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
Synonyms	EMCN, EMCN2, MUC14, Endomucin-2, Mucin-14, Gastric cancer antigen Ga34
Reference Data

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