WEE1 pSer642 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1:500~1:1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1 (KLH-conjugated)
Specificity	The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	100 kDa
Gene Name	WEE1 G2 checkpoint kinase
Database Link	Entrez Gene 7465 Human UniProt ID P30291
Background	Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Synonyms	Wee1A kinase, WEE1hu
Reference Data

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