MDM2 pSer186/Ser188 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF
Recommend Dilution	Immunofluorescence: 1:100~1:200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around phosphorylation site of Ser186/Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2 (KLH-conjugated)
Specificity	The antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	55 kDa
Gene Name	MDM2 proto-oncogene
Database Link	Entrez Gene 4193 Human UniProt ID Q00987
Background	This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues
Synonyms	p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2
Reference Data

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