Munc18 1 (STXBP1) pThr574 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1/1000-1/5000. Immunohistochemistry: 1/100-1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from C-terminal domain of Human STXBP1 protein containing Phosphorylated Thr574
Specificity	Reacts with Human 67 kDa Unc18-1 phosphorylated at Threonine 574.
Formulation	0.1M Tris, 0.1M Glycine and 2% Sucrose State: Purified State: Lyophilized purified antibody Preservative: None
Reconstitution Method	Restore in distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store the antibody at -20°C. Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	syntaxin binding protein 1
Database Link	Entrez Gene 6812 Human UniProt ID P61764
Background	Unc18-1, 2, and 3 (syntaxin binding proteins 1-3, STXBP1-3, UNC18-a-c, MUNC18-1-3) are chaperone molecules that block syntaxin interactions with cognate SNARE (soluble NSF attachment protein (SNAP) receptor) proteins and regulate exocytosis. Unc18-1-3 mRNA is present in RBL-2H3 mast cells, mouse bone marrow derived mast cells (BMMC), and platelets. Unc18-1 Ser-313 is a protein kinase C phosphorylation site and Thr-574 is a cyclin-dependent kinase 5 phosphorylation site that regulates Unc18-1/syntaxin1A interactions. Unc18-1 is phosphorylated on Ser-313 in response to phorbol ester treatment in adrenal chromaffin cells. Unc18-2 colocalizes with syntaxin 3 at the apical plasma membrane of intestinal, proximal tubule, and collecting duct epithelial cells.
Synonyms	Unc-18 homolog, Unc-18-1, Unc-18A, N-Sec1, Syntaxin-binding protein 1
Reference Data

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