Tryptophan 5-hydroxylase 2 (TPH2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: Preliminary experiments gave an approx 85kDa band in Mouse fetal Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.9kDa according to NP_775567.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Paraffin Sections: 0.5-2 µg/ml. In paraffin embedded Mouse Brain stem shows staining of Raphe nucleii serotonergic neurons consistent with observation by different antibodies.
Reactivity	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_775567.2.
Specificity	This antibody recognizes Mouse Tryptophan 5-hydroxylase 2. Other species not tested.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Antigen Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tryptophan hydroxylase 2
Database Link	Entrez Gene 216343 Mouse UniProt ID Q8CGV2
Synonyms	Tryptophan 5-monooxygenase 2, Neuronal tryptophan hydroxylase, NTPH
Reference Data

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