PTCH1 (266-280) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 1-3 μg/ml. Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1). Immunofluorescence: 5 µg/ml. Strong expression of the protein seen in the cytoplasm of HeLa cells.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence (266-280) according to NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1.
Specificity	This antibody is expected to recognize all reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	patched 1
Database Link	Entrez Gene 5727 Human UniProt ID Q13635
Synonyms	PTCH, PTC1, PTC, Patched 1
Reference Data

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