Proteasome subunit beta type 4 (PSMB4) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/16000 (Detection Limit). Western blot: 0.3-1.0 μg/ml. Approx 25kDa band observed in lysates of cell lines NIH3T3, HEK293, HeLa, Jurkat and K562. Primary incubation was 1 hour. Immunohistochemistry on Paraffin Sections: 3.75 µg/ml (Human Skin).
Reactivity	Bovine, Canine, Human, Mouse, Rabbit, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_002787.2.
Specificity	This antibody recognizes PSMB4.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	proteasome subunit beta 4
Database Link	Entrez Gene 5692 Human UniProt ID P28070
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.
Synonyms	Proteasome subunit beta type-4, Proteasome beta chain, HsBPROS26, PROS-26, Macropain beta chain, Proteasome chain 3, HsN3
Note	Calculated Molecular Weight (mature protein): 24.3kDa according to aa46-264 of NP_002787.2.
Reference Data

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