Melatonin Receptor 1B (MTNR1B) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection limit).  Western blot: Preliminary experiments gave approx .48kDa and 37kDa bands in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_005950.1. The 48kDa and 37kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?  Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. In paraffin embedded Cerebellum shows membranous staining primarily in Purkinje cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide with sequence from the C Terminus of Human MTNR1B (NP_005950.1).
Specificity	This antibody detects MTNR1B at C-term
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	melatonin receptor 1B
Database Link	Entrez Gene 4544 Human UniProt ID P49286
Background	MTNR1b is a Melatonin Receptor that mediates the effects of melatonin in the retina and the photoperiodic control of neuroendocrine functions. It may also be involved in melatonin-induced immune enhancement.MTNR1b has been reported in adipose, brain, eye, and ovary. An EST has been isolated from an eye tissue library.
Synonyms	MTNR1B
Reference Data

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