Sterol carrier protein 2 (SCP2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection limit). Western blot: Preliminary experiments gave an approx 50kDa band in Human Liver lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0kDa according to NP_001007099.1 and of 6.7kDa according to NP_002970.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported? Immunohistochemistry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Liver shows speckled cytoplasm staining in hepatocytes.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from an internal region of human SCP2 (NP_001007099.1; NP_002970.2; NP_001180528.1; NP_001180529.1; NP_001180546.1).
Specificity	This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 of SCP2 (NP_002970.2, NP_001007099.1, NP_001180529.1, NP_001180528.1 and NP_001180546.1 respectively).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	sterol carrier protein 2
Database Link	Entrez Gene 6342 Human UniProt ID P22307
Background	This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. The full-length nature of all transcript variants has not been determined. [provided by RefSeq]
Synonyms	SCP-2, SCP-X, SCP-chi, Sterol carrier protein 2, Sterol carrier protein X, NSL-TP
Reference Data

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