Dopamine beta Hydroxylase (DBH) (437-448) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection limit). Western blot: 1-3 µg/ml. Detects band of Approx 70kDa in lysates of cell line Jurkat. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch. Immunohistochemistry on Paraffin Sections: 5 µg/ml in Paraffin embedded Human Adrenal Gland.
Reactivity	Bovine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide (-DNHYSPHFQEIR) from the internal region of Human DBH (NP_000778.3).
Specificity	This antibody recognizes DBH (437-448). This batch is not expected to cross-react with Mouse and Rat in Western blot.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	dopamine beta-hydroxylase
Database Link	Entrez Gene 1621 Human UniProt ID P09172
Background	DBH is a member of the copper type II, ascorbate-dependent monooxygenase family, which catalyzes the conversion of dopamine to norepinephrine, a key neurotransmitter in the central and peripheral nervous systems. The effects of DBH plasma levels have been a focus area for both neurological and psychiatric disorders, including Parkinsons disease, hypertension, depression, schizophrenia, drug abuse and stress.
Synonyms	DBH, Dopamine beta-monooxygenase, DBM, DOPO
Reference Data

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