Myelin Basic Protein (MBP) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/2000-1/5000 for Western Blots. 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: This antibody was analyzed by Immunohistochemistry (at a 1/2000 dilution) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Synthetic peptide KLH conjugated corresponding to a region of the MBP gene product shared between the Human (NP_002376) and Mouse (NP_034907) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column.
Specificity	Recognizes Myelin Basic Protein (MBP).
Formulation	10mM PBS, pH 7.2 State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction Preservative: 0.02% (w/v) Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column
Conjugation	Unconjugated
Storage Condition	Store undiluted in the dark at 2-8°C. DO NOT FREEZE!
Gene Name	myelin basic protein
Database Link	Entrez Gene 17196 MouseEntrez Gene 4155 Human UniProt ID P02686
Background	The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.
Synonyms	MBP
Reference Data

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