Histone H3 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunocytochemistry: 1/100. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Immunoprecipitation. Western Blot: 1/300.
Reactivity	Bovine, Human
Host	Sheep
Clonality	Polyclonal
Immunogen	Intact Calf Histone H3 at 15.4 kD, 136 amino acids, complexed with RNA.
Specificity	Recognizes Histone H3. Minimal cross-reactivity with linker Histone H1 and no cross-reactivity with Histone core proteins H2A, H2B, or H4.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Synonyms	H3 Histone family, HIST1H3
Reference Data

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