PACE4 (PCSK6) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: Detection Limit: 1/32000. Western Blot: Preliminary experiments gave an approx 40-45 kDa band in Human Brain (Cerebellum) lysates after 0.2 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kDa according to NP_002561.1. The 40-45 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. In Paraffin Embedded Human Skeletal Muscle shows striated staining in alternating fibres.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1.
Specificity	Recognizes PCSK6 / PACE4. This antibody is expected to recognize reported isoforms a (NP_002561.1), b (NP_612192.1), g (NP_612193.1) and h (NP_612194.1).
Formulation	Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	proprotein convertase subtilisin/kexin type 6
Database Link	Entrez Gene 5046 Human UniProt ID P29122
Background	PACE4 belongs to the peptidase S8 family. Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif. Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Synonyms	SPC4
Reference Data
Protein Families	Druggable Genome, Protease, Secreted Protein

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