DR5 (TNFRSF10B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human sTRAIL Receptor-2 by Direct ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sTRAIL Receptor-2. Sandwich ELISA: To detect Human sTRAIL Receptor-2 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Human sTRAIL Receptor-2 (AP31096BT-N or AP31096BT-S) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sTRAIL Receptor-2. Western Blot: To detect Human sTRAIL Receptor-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL Receptor-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) 14,9 kDa recombinant Human soluble TRAIL Receptor-2.
Specificity	Recognizes sTRAIL Receptor-2.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing an immobilized Human sTRAIL Receptor-2 matrix
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor receptor superfamily member 10b
Database Link	Entrez Gene 8795 Human UniProt ID O14763
Background	Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.
Synonyms	TNFRSF10B, DR5, KILLER, TRICK2, ZTNFR9, Death receptor 5, TRAIL receptor 2, TRAIL-R2
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity, p53 signaling pathway

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