Tnni3 pSer43 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1:1000. Immunolabeling is greatly decreased with lambda -phosphatase treatment.
Reactivity	Canine, Human, Mouse, Primate, Rat, Xenopus, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 43 of mouse troponin I, cardiac (cTnI)
Specificity	Specific for the ~25k cardiac troponin I protein phosphorylated at Ser 43.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BS A and 50% glycerol State: Aff - Purified State: Liquid ig fraction
Purification	Affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	troponin I, cardiac 3
Database Link	Entrez Gene 21954 Mouse UniProt ID P48787
Background	Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) an d fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important duri ng heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser 43 and Ser 45 of cardiac troponin I plays a substantial role in the PKC- mediated depression. (Montgomery et al, 2002).
Synonyms	TNNI3, TNNC1
Reference Data

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