PTPH1 (PTPN3) pSer459 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/1000.
Reactivity	Bovine, Canine, Human, Mouse, Primate, Sheep
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser459 of human PTPH1
Specificity	Specific for ~104k PTPH1 protein phosphorylated at Ser 459. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with λ phosphatase.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Purification	Affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, non-receptor type 3
Database Link	Entrez Gene 5774 Human UniProt ID P26045
Background	Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38 γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38 γ and PTPH1 protein expression, and there is a coupling of increased p38 γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylati on of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).
Synonyms	Protein-tyrosine phosphatase H1, PTP-H1
Reference Data

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