CCL27 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	Sandwich ELISA: To detect Human CTACK by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTACK (Cat.-No AP23787BT-N or AP23787BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTACK. Western Blot: To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunofluorescence: This antibody stained HeLa cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant Human CTACK.
Specificity	Recognizes Human CTACK. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing an immobilized Human CTACK matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 27
Database Link	Entrez Gene 10850 Human UniProt ID Q9Y4X3
Background	CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues.
Synonyms	C-C motif chemokine 27, Small-inducible cytokine A27, ILC, SCYA27, Skinkine, CTACK
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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