SATB1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit).  Western Blot: 0.3-1 µg/ml. Experiments gave bands at approx. 110kDa and 85kDa in lysates of cell line MOLT4 after 0.3 µg/ml antibody staining. The observed 110kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol. 1998 Apr 20;141(2):335-48.; PMID: 9548713). This protein has a calculated MW of 86.0kDa according to NP_002962.1. Immunohistochemistry on Paraffin Sections: 3-5 μg/ml. In paraffin embedded Human Thymus shows select nuclear staining.
Reactivity	Bovine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide sequence from the internal region of Human SATB1 (NP_002962.1).
Specificity	This antibody recognizes SATB1.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purfied IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	SATB homeobox 1
Database Link	Entrez Gene 6304 Human UniProt ID Q01826
Synonyms	SATB1
Reference Data

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