CYB5R3 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western Blot: 0.01-0.03 µg/ml. Approx 37kDa band observed in Human Placenta, Testis and Umbilical Cord lysates.  Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Adrenal Gland shows heavy vesicular/granular staining in the cytoplasm of medular cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminus of human CYB5R3 (NP_000389.1; NP_001123291.1; NP_001165131.1).
Specificity	This antibody is expected to recognize all three reported isoforms (NP_000389.1, NP_001123291.1 and NP_001165131.1).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction. Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cytochrome b5 reductase 3
Database Link	Entrez Gene 1727 Human UniProt ID P00387
Background	CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and contains 1 FAD-binding FR-type domain. There are two isoforms, NADH-cytochrome b5 reductase 3 membrane bound form and NADH-cytochrome b5 reductase 3 soluble form. Its functions include desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1) in which the enzyme is only deficient in erythrocytes with a mild cyanosis; type 2 (HM2), in which the enzyme is completely deficient; type 3 (HM3) where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied with mental retardation and neurological impairment.
Synonyms	NADH-cytochrome b5 reductase 3, B5R, DIA1, Diaphorase-1
Note	Calculated Molecular Weight: 38.2kDa (NP_001165131.1).
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Amino sugar and nucleotide sugar metabolism

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