SSH3BP1 (ABI1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect Abi-1 in cells and tissues. Immunohistochemistry on paraffin sections: At 0.5-1μg/ml to detect Abi-1 in formalin fixed and paraffin embedded tissues. Immunohistochemistry on frozen sections: At 0.5-1μg/ml to detect Abi-1 in formalin or acetone fixed tissues. Immunocytochemistry: At 0.5-1μg/ml to detect Abi-1 in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminal of human ABI-1
Specificity	This antibody detects ABI1 / SSH3BP1 (C-term).
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	abl interactor 1
Database Link	Entrez Gene 11308 MouseEntrez Gene 79249 RatEntrez Gene 10006 Human UniProt ID Q8IZP0
Background	ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA,but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins.ABI1 plays a role in the leukemogenesis by translocating to MLL.
Synonyms	Abl interactor 1, Abelson interactor 1, Abi-1, e3B1, Spectrin binding protein, Nap1-binding protein, Abl-binding protein 4, AblBP4
Reference Data

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