Alkaline Phosphatase (ALPL) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect ALPL in cells and tissues. The detection limit for ALPL is approximately 2.5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry on Paraffin Sections: At 1-2 μg/ml to detect ALPL in formalin fixed and paraffin embedded tissues. Antigen Retrieval by Heat is required.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence at the N-terminal of Human ALPL (21-35)
Specificity	This antibody detects Alkaline phosphatase / ALPL at N-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal and 0.05mg Sodium Azide State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	alkaline phosphatase, liver/bone/kidney
Database Link	Entrez Gene 11647 MouseEntrez Gene 25586 RatEntrez Gene 249 Human UniProt ID P05186
Background	Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Synonyms	AP-TNAP, TNSALP
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Folate biosynthesis, Metabolic pathways

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