NSE (ENO2) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect NSE in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect NSE in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immuncytochemistry. Immunprecipitation.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide mapping at the C-terminus of NSE of human origin
Specificity	This antibody detects Neuron specific enolase at C-term.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	enolase 2
Database Link	Entrez Gene 13807 MouseEntrez Gene 24334 RatEntrez Gene 2026 Human UniProt ID P09104
Background	NSE (neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
Synonyms	NSE, ENO2, Enolase 2,Neural enolase, Gamma-enolase
Reference Data
Protein Pathways	Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation

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