DUT Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	Western blot: At 0.5-1μg/ml with the appropriate system to detect dUTPase in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect dUTPase in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunoprecipitation.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence mapping at the middle region of human dUTPase
Specificity	This antibody detects DUT in the middle. No cross reactivity with other roteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg himerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	deoxyuridine triphosphatase
Database Link	Entrez Gene 110074 MouseEntrez Gene 497778 RatEntrez Gene 1854 Human UniProt ID P33316
Background	Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low ntracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. DUTPase ctivity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and UTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the ytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E. coli UTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial orms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the se of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this nzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.
Synonyms	dUTPase, dUTP pyrophosphatase
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Pyrimidine metabolism

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