Fas (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: Use at 1 µg/ml with the appropriate system to detect FAS in cells and tissues. Immunohistochemistry on Frozen Sections: Use at 1-2 µg/ml to detect FAS in Formalin or Acetone fixed tissues. Immunohistochemistry on Paraffin Sections: Use at 1-2 µg/ml to detect FAS in Formalin Fixed and Paraffin Embedded tissues. Antigen Retrieval by heat is required.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminal of Rat FAS, different from the related Mouse sequence by seven amino acids.
Specificity	Recognizes FAS. No cross reactivity with other proteins.
Formulation	0.9 mg NaCl, 0.2 mg Na2HPO4 State: Aff - Purified State: Lyophilized purified IgG fraction Stabilizer: 5 mg BSA Preservative: 0.05 mg Thimerosal and 0.05 mg Sodium Azide
Reconstitution Method	Restore with 0.2 ml of distilled water to yield a concentration of 0.5 mg/ml.
Concentration	0.5 mg/ml (after reconstitution)
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Fas (TNF receptor superfamily member 6)
Database Link	Entrez Gene 246097 RatEntrez Gene 14102 Mouse UniProt ID P25446
Background	FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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