Hsp60 (HSPD1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 0.5-1µg/ml with the appropriate system to detect HSP60 in cells and tissues. Immunohistochemistry on paraffin sections: At 0.5-1µg/ml to detect HSP60 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry on frozen sections. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide mapping at the middle region of human HSP60
Specificity	This antibody detects HSP60 in the middle.
Formulation	50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4 State: Aff - Purified State: Lyophilzed Ig fraction
Reconstitution Method	1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein family D (Hsp60) member 1
Database Link	Entrez Gene 15510 MouseEntrez Gene 63868 RatEntrez Gene 3329 Human UniProt ID P10809
Background	Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNFsecretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Synonyms	HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	RNA degradation, Type I diabetes mellitus

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