GFAP (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 0.1-0.5 µg/ml. Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence at the C-terminal (417-432aa) of Human GFAP.
Specificity	This antibody detects GFAP at C-term. No cross reactivity with other proteins.
Formulation	0.2 mg Na2HPO4, 0.9 mg NaCl State: Aff - Purified State: Lyophilized purified IgG fraction Stabilizer: 5 mg BSA Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
Reconstitution Method	0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	glial fibrillary acidic protein
Database Link	Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human UniProt ID P14136
Background	GGlial fibrillary acidic protein (GFAP) is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. GFAP has been located in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. GFAP has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.
Synonyms	Glial Fibrillary Acidic Protein
Reference Data
Protein Families	ES Cell Differentiation/IPS

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